New microscope breaks light microscopy resolution barrier

DePurpereWolf

New microscope breaks light microscopy resolution barrier

A new laser-equipped microscope at IU Bloomington's Light Microscopy Imaging Center makes it possible to examine biological samples with unprecedented detail in three dimensions.

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The imager is exceptionally fast in collecting images of a biological specimen, and this speed enables scientists to gather crucial data. The device uses laser light of four different colors to illuminate samples, while four extremely sensitive digital cameras capture images every 10 milliseconds at the imager's speediest setting. The device can produce as many as 5,000 full-color images per minute for its major task of producing high-resolution images. Known as a "structured illumination" microscope, the device will help IU scientists attain a better understanding of how proteins are distributed inside cells with unprecedented resolution.

Most high-technology light microscopes reach the limits of resolution at 250-300 nanometers -- the diameter of a small bacterial cell. The new OMX microscope IU has acquired can produce clear images down to 100 nanometers in the lateral dimension. Resolution along the z-axis (perpendicular, or coming out of the page) is somewhat lower but still tremendously improved relative to previous technologies.

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Lees verder: http://www.physorg.com/news200761303.html

Bron: Phys org

Wouter_Masselink

Kwijl! hebben hebben hebben.

Al deze manieren om de diffractie limiet te doorbreken gaan mij redelijk boven de pet. Deconvolutie snap ik nog wel, maar al dit andere mogelijke manieren zijn voor mij op het moment meer abracadabra.

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New microscope breaks light microscopy resolution barrier

New microscope breaks light microscopy resolution barrier

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