) any help? Pretty please...Laatste berichten
-
22:27
positie 1
-
21:34
[scheikunde] Kan chloorgas de geleiding van elektriciteit belemmeren? 8
-
21:15
Weerfrustratie 9
-
18:24
Schroefdraad berekening 5
-
18:08
geen minkowski-ruimte toch? Doe ik dit nou fout? 15
-
14:16
do-re-mi-fa-so vliegtuigen 7
-
14:16
Kunnen quantum Zonnecellen 190% quantum efficiënt zijn 3
-
13:57
De Euro Nederlandse 100 qubit computer komt eraan
-
10:42
projectiel 8
-
10:37
Verschil tussen deze 2 vragen 5
-
09:25
[scheikunde] berekeningen labo vitamine c bepaling 1
-
22 apr
[wiskunde] rode en witte ballen verdelen 8
-
22 apr
Rotatie van het heelal 41
-
22 apr
Muntje opgooien 14
-
21 apr
Reactiviteit silyl enol ethers 1
-
21 apr
Criterium voor vochtretentie
-
21 apr
speciale rel. theorie 5
-
21 apr
Vogels in de stad zijn goede klussers
-
21 apr
Ervaringen met "herontdekkingen" 15
-
20 apr
Herleiden afmetingen vanaf een foto 19
Nieuwsberichten
-
04 mar
Een nieuw soort magnetisme: altermagnetisme
-
31 okt
AI kan via stem diabetes vaststellen 11
-
21 okt
Einstein krijgt wéér gelijk 45
-
07 feb
witter dan wit 20
-
19 jun
irrigatie en de aardas
Nefelometrie
Moderator: ArcherBarry
Forumregels
(Middelbare) school-achtige vragen naar het forum "Huiswerk en Practica" a.u.b.
Zie eerst de Huiswerkbijsluiter
(Middelbare) school-achtige vragen naar het forum "Huiswerk en Practica" a.u.b.
Zie eerst de Huiswerkbijsluiter
-
- Berichten: 26
Nefelometrie
Ik moet voor het vak immunologische technieken iets vertellen over nefelometrie, in verband met antiestoffen en immunoglobulines. Ben niet zo'n google wonder en vind dus veel te veel rotzooi(inschrijvingen voor cursi scheikunde enzo ) any help? Pretty please...
) any help? Pretty please...-
- Berichten: 111
Re: Nefelometrie
Essential Immunology (Blackwel Scientific Publications):
"The nephelometric assay for antigen
If antigen is added to a solution of excess antibody, the amount of complex which can be assessed by forward light scatter in a nephelometer is linearly related to the concentration of antigen. With the ready availability of a wide range of monoclonal antibodies which faciliate the standardization of the metyhod, nephelometry is replacing SRID for the estimation of immunoglobins, C3, CRP, etc. in those favored laboratories which can sport the appropriate equipment. Very small samples own in the range 1-10µl can be analyzed. Turbidity of the sample can be a problem; blanks lacking antibody can be deducted but a more satisfactory solutiond is to follow the rate of formation of complexes which is proportional to antigen concentration since this obviates the need for a separate blank. Reference back to the percipitin curve reminds us that a given amount of complex can be formed on either the ascending or descending parts of the curve; to ensure that ther value for antigen was obtained in antibody excess, it is wise to run a further control in which additional antigen is included.
Nonpercipitating antibodies can be detected by nephelometry
The small aggregates formed when dilute solutions of antigen and antibody are mixed create a cloudiness or turbidity which can be measured by low angle forward scattering of aan incident light source (nephelometry). Greater sensitivity can be obtained ny using monochromatic light from a laser and by adding polyethylene glycol to the solution so that the aggregate size is increased. In practice, nephelometry is applied more to the detection of antigen than antibody and this will be dealt with in a later section."
"The nephelometric assay for antigen
If antigen is added to a solution of excess antibody, the amount of complex which can be assessed by forward light scatter in a nephelometer is linearly related to the concentration of antigen. With the ready availability of a wide range of monoclonal antibodies which faciliate the standardization of the metyhod, nephelometry is replacing SRID for the estimation of immunoglobins, C3, CRP, etc. in those favored laboratories which can sport the appropriate equipment. Very small samples own in the range 1-10µl can be analyzed. Turbidity of the sample can be a problem; blanks lacking antibody can be deducted but a more satisfactory solutiond is to follow the rate of formation of complexes which is proportional to antigen concentration since this obviates the need for a separate blank. Reference back to the percipitin curve reminds us that a given amount of complex can be formed on either the ascending or descending parts of the curve; to ensure that ther value for antigen was obtained in antibody excess, it is wise to run a further control in which additional antigen is included.
Nonpercipitating antibodies can be detected by nephelometry
The small aggregates formed when dilute solutions of antigen and antibody are mixed create a cloudiness or turbidity which can be measured by low angle forward scattering of aan incident light source (nephelometry). Greater sensitivity can be obtained ny using monochromatic light from a laser and by adding polyethylene glycol to the solution so that the aggregate size is increased. In practice, nephelometry is applied more to the detection of antigen than antibody and this will be dealt with in a later section."
